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The purpose of the present study was to evaluate the effects of resveratrol and shikonin, two natural agents, on the induction and maintenance of osteosarcoma cell apoptosis. The results showed that resveratrol and shikonin were able to induce apoptosis in osteosarcoma cell lines in a dose-dependent manner, and induced a more potent cytotoxic effect in the MG63 cell line. DAPI staining revealed that resveratrol induced a slight nuclear fragmentation in MG63 cells, while the treatment of MG63 cells with shikonin induced typical apoptotic nuclear changes, such as chromatin condensation and increased fragmentation. Following treatment, the MG63 cells exhibited an increase in the expression of Bax, Fas, caspase-3 and p53, but an decrease in Bcl-2 expression. In addition, the activities of caspase-3 were significantly enhanced after incubation with resveratrol and shikonin. Finally, the treatment of MG63 cells with resveratrol and shikonin led to a significant increase in the TUNEL-positive cells and the cleavage of poly (ADP-ribose) polymerase. Taken together, the findings suggest that resveratrol and shikonin may act on cancer cells, affecting apoptosis-related pathways. Thus, these natural agents may be effective against osteosarcoma cells and provide a basis for the development of potential therapeutic agents.Q: How to get the value of a radio button in a different page and use it on the main page in asp.net Ntfs Recovery Wizard Patch With Serial Key [Updated] The most crucial factor in the operation of your PC is the motherboard itself. In case of an error, this hardware can cause all sorts of troubles. At times, a kernel error can become the primary cause of troubles in the background, making it really hard to ascertain the real source of your problems. 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